Composite

Part:BBa_K3160021:Design

Designed by: chi xu   Group: iGEM19_XHD-WS-Wuhan-A   (2019-10-16)


MCherry-miR-21-17 sensor


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 566
    Illegal XhoI site found at 965
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 700
    Illegal AgeI site found at 79
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The mcherry-miR-21-17 sensor was controlled by EF-1α promoter (EF-1 alpha -mcherry-miR-21-17). The plasmids of EF-1 alpha -mcherry-miR-21-17 were transfected into different cell lines (one is human gastric epithelial cell line. The others are gastric cancer cell lines).We measuresd the fluorescence of mcherry in different cells transfected with EF-1 alpha -mcherry-miR-21-17 for 24 h by plate reader (SpectraMax i3), which analyses the potential value of EF-1 alpha -mcherry-miR-21-17 to measure the expression of miR-21 and miR-17.


Source

We ordered this part DNA from a synthesis company

References